shuttling plasmids

S. L. Rennie rennie at bldghsc.lan1.umanitoba.ca
Thu Jun 29 13:03:53 EST 1995


In article <JCHO.2.0 at bio1.lan.mcgill.ca>, JCHO at bio1.lan.mcgill.ca (John
Cho) wrote:

> I have been attempting to shuttle a plasmid out of my yeast cell back into E.
> coli with little success. The method I have been using involves glass beads (
> Nucleic Acids Research, 1990, 18, 5319) - this protocol is said to be better 
> than the Hoffman and Winston protocol. I, however, have had success with 
> this protocol only once. What's the secret??
> 
> John Cho
> McGill University
-----------------------------
We just use Hoffman and Winston and EP the plasmid into E.coli.  This is Very
quick and efficient, short of figuring out how that Double Zap method works
I'm not sure what would be more effective - - unless you want higher yield of
plasmid??

Just my $.02
Sharon



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