shuttling plasmids

John Cho JCHO at
Wed Jun 28 11:14:23 EST 1995

I have been attempting to shuttle a plasmid out of my yeast cell back into E.
coli with little success. The method I have been using involves glass beads (
Nucleic Acids Research, 1990, 18, 5319) - this protocol is said to be better 
than the Hoffman and Winston protocol. I, however, have had success with 
this protocol only once. What's the secret??

John Cho
McGill University

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