recovering plasmids from yeast

Brent A. Spoth bas8v at dayhoff.med.Virginia.EDU
Fri Jun 30 07:05:49 EST 1995


This is in response to problems with recovering plasmids from budding 
yeast.  We have started using the prot'l from NAS V20 #14.  This is essentially 
the standard glass bead method with a ammonium acetate ppt. thrown in.  This 
extra step seems to get rid of most of the inhibitory agents and GREATLY 
increases the efficiency of the process.  I highly recommend giving it a try!

Brent
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