S. pombe gene disruption

[Preston Garrison]

Dear pombe gurus,

We are planning our first gene disruption in pombe. Available restriction 
sites make it convenient to have about 100 bp of homology on one end and 
400 bp on the other. Does this sound like a workable design or is the 
100 bp end going to cause us problems?

Thanks in advance,

Preston G.

Preston Garrison                "Hey, sister, that's astute, 
garrisonp at uthscsa.edu            why don't we get together
Biochem. Dept.                   and call ourselves an Institute?"
Univ of Texas Health Sci Ctr                       Paul Simon
San Antonio, Tx 78284-7760 USA
210-567-3702