How to catch calcium binding proteins?

Jochen Strayle Biochemistry Stuttgart-Germany jochen.strayle at PO.UNI-STUTTGART.DE
Mon Mar 6 04:15:33 EST 1995


Hi out there

I need some help from experienced biochemists. I have a protein with maybe 
week calcium binding sites ( no EF-hands). And I want to show that this protein 
binds calcium, at least. Has anybody experiences with gelshift experiments, 
dyes or something else to detect low affinity calcium binding sites. I don«t know 
if it«s been tried.
If it does, let me know.
Thanks,
Jochen

e-mail:  jochen.strayle at po.uni-stuttgart.de





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