yeast chromosome isolation

Kevin Piers klpiers at U.WASHINGTON.EDU
Wed Mar 8 18:10:17 EST 1995


Good Day Netters,

I am preparing to isolate yeast chromosomes for the purpose of CHEF 
analysis and was wondering about good protocols to use for doing so.  I 
have the basic protocol from Maniatis.  Does this work well or are there 
some "secret" tricks that need to be done to ensure success.  Also, the 
protocol suggests using zymolyase 5000.  Can one substitute zymolyase 
100,000 for this or would that be detrimental?  Any tidbits of info are 
welcome.

Thanks,

Kevin Piers
Dept. of Microbiology
University of Washington
Seattle WA 98195



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