Need meth. to distinguish exo/endo glucanase

BiotecBurd biotecburd at
Wed Mar 8 08:16:28 EST 1995

In my work I've looked at enzyme specificity by combining reducing sugar
analysis with HPLC.  The breakdown products can be identified using a
sugar pak column from Millipore, however this requires refractometry.  You
may try a glucose oxidase test to measure glucose liberation by the
exoglucanase coupled to a reducing sugar assay.

Dave Burden
Biotechnology Training Institute

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