Need meth. to distinguish exo/endo glucanase

biotecburd at biotecburd at
Wed Mar 8 08:11:04 EST 1995

Greetings.  Obviously reducing sugar assays indicate hydrolysis, but not
location.  In my work, the only way of identifying location of glucanase
cleavage is by combining reducing sugar assays with a glucose oxidase test
or running the enzyme assay over a sugar separation column on a HPLC.  I
routinely looked a residual sugars in fermentation broths using a Sugar
Pak column by Millipore.  The only headache with sugar analysis by HPLC is
that it requires refractometry which is a pain.

I hope this is some help.

Dave Burden
Biotechnology Training Institute

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