Red yeast - red DNA

Michael Lichten lichten at HELIX.NIH.GOV
Fri Mar 10 09:36:40 EST 1995


Anthony Tomlinson writes,

>Does anyone know how to get rid of the red Ad. pigment that copurifies
>with genomic DNA in rapid prep methods?  Alternatievly, is is worth
>trying to
>get rid of it?  I need the DNA for PCR, restriction and ligation.  Is the
>pigment
>inhibitory to these?  I've tried Nick coloumns but lost half of the DNA
>and didn't get
> all of the red out.
>
Try Visine?  Seriously, though, I have never had a problem with restricting
red DNA.  Never tried ligation.


Michael Lichten
lichten at helix.nih.gov






More information about the Yeast mailing list