Two hybrid Q.
rafael at corona
Tue Mar 14 15:22:27 EST 1995
I'm trying the two hybrid method for detecting interacting proteins in yeast.
I have seen that the proteins to be tested are disposed in the C-terminal
of the gal4 protein domains (both). I mean, the fusions are always in the
Because I'm interested in the interaction with the N-terminal end of my
protein, can I use the fusion in the other way, N*Myprotein-gal4domain*C?
Have anyone tried that? Do you think it would work?
Rafael Maldonado "You gave to a boy the job
Department of Human Genetics of a man. He's dead."
University of Utah
Salt Lake City, Utah 84112. USA. John Wayne
Rafael at genetics.med.utah.edu
Rafael at corona.med.utah.edu "No por mucho madrugar, amanece
Tlf: 801-581-4429 mas temprano"
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