immunofluorescence background

Dave Gilbert gilbertd at VAX.CS.HSCSYR.EDU
Fri Mar 17 06:53:59 EST 1995


Does anyone have any tricks to reduce apparently non-specific association
of polyclonal antibodies to yeast cells.  We have tried blocking steps with
serum, however, our immunofluorescent signals with antibody are still only
marginally above signals obtained with pre-immune serum.

Thanks.

Dave Gilbert
Department of Biochemistry and Molecular Biology
SUNY Health Science Center
750 E. Adams St.
Syracuse, N.Y. 13210
Tel:(315) 464-8723
FAX:(315) 464-8750





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