immunofluorescence background

Anne Spang spang at vms.biochem.mpg.de
Sun Mar 19 12:04:27 EST 1995


Hi Dave!

Have you tried to increase the salt concentration in your buffers? You
might get very specific signals up to 300 mM NaCl? Another possibilty
would be, using different fixation times. Sometimes, short fixation times
increase the non-specific background.
Another question: how did you affinity purify your antibodies??

Hope this helps!
Anne



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