Transforming E. coli with Yeast DNA... HOW!?
EGUARINOS at mvax.cbm.uam.es
Tue Mar 21 11:15:14 EST 1995
I'm trying to check some yeast transformants by transforming E. coli with
their DNA. The trouble is that I don't get any transformant of E. coli.
How can I prepare the yeast DNA to get some transformants?
It's important to quantify the DNA? If so, which is the optimal
Is there any trick to improve the transformation?
If someone can help me, please send the answer to my personal account. I
will post a summary of the answers I receive.
Thanks a lot,
Centro de Biologia Molecular "Severo Ochoa"
Universidad Autonoma de Madrid-CSIC
Cantoblanco. 28049 Madrid
Fax : 34-1-3974799
e-mail: eguarinos at mvax.cbm.uam.es
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