b-galactosidase

Colin MacD macdiarm at sbsu1.auckland.ac.nz
Wed Mar 22 16:48:06 EST 1995


> Since this is a plant molecular bio lab, I have decided to use the
> b-glucuronidase (GUS) gene instead - seems to be superior to b-gal in that
> there is no activity in yeast, and can be assayed in the same way.  As
> well, we have the (modified for euk expression) gene in pUC.
> > FYI the papers are PNAS 83, 8447-8451 and Curr Genet 17, 261-264.
> > 
> > By the way, anyone tried the jellyfish 'green fluorescent protein' as a
> marker gene in yeast?
> > 

I guess I implied above that yeast has a b-gal activity - cerevisiae
doesnt of course, but my friend who works on K lactis tells me gus is the
gene of choice for this species (which does have endogenous b-gal).

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