Transforming E. coli with Yeast DNA... HOW!?
Bert.Popping at durham.ac.uk
Thu Mar 23 07:07:09 EST 1995
could you give some parameters for the electroporation method ?
Do you use a single pulse ? kV, ohm, capacity ?
Or perhaps you can refer to a publication where the method is described.
We're using the method described by Marcil_R, Higgins_DR:
Direct transfer of plasmid DNA from yeast to E.coli by
electroporation, Nucl. Acids Res, 20/4, p 917 (1992).
It seems to work for some yeast strains but not for others. But
the method is *very* simple and worth a trial !
Bert Popping http://www.dur.ac.uk/~dbl1bp
Dept. Biol. Sci. http://www.dur.ac.uk/~dbl0zz3
University of Durham
Durham, DH1 3LE
Phone +44 191 374 7576
Fax +44 191 374 2417
E-Mail Bert.Popping at durham.ac.uk
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