Transforming E. coli with Yeast DNA... HOW!?

Bert Popping Bert.Popping at durham.ac.uk
Thu Mar 23 07:07:09 EST 1995


Dear Susan,

could you give some parameters for the electroporation method ?
Do you use a single pulse ? kV, ohm, capacity ?
Or perhaps you can refer to a publication where the method is described.

We're using the method described by Marcil_R, Higgins_DR:
Direct transfer of plasmid DNA from yeast to E.coli by 
electroporation, Nucl. Acids Res, 20/4, p 917 (1992).

It seems to work for some yeast strains but not for others. But
the method is *very* simple and worth a trial !


Bert Popping             http://www.dur.ac.uk/~dbl1bp
Dept. Biol. Sci.         http://www.dur.ac.uk/~dbl0zz3
University of Durham
Durham, DH1 3LE
England
Phone +44 191 374 7576
Fax   +44 191 374 2417
E-Mail Bert.Popping at durham.ac.uk





More information about the Yeast mailing list