Transforming E. coli with Yeast DNA... HOW!?

Colin MacD macdiarm at sbsu1.auckland.ac.nz
Thu Mar 23 16:30:04 EST 1995


In article <3kqgju$ikj at riscsm.scripps.edu>, SLForsburg
<susan_forsburg at qm.salk.edu> wrote:

> Esther Guarinos <EGUARINOS at mvax.cbm.uam.es> wrote:
> >
> >      I'm trying to check some yeast transformants by transforming E.
coli with 
> > their DNA. The trouble is that I don't get any transformant of E. coli.
> > 
> >      How can I prepare the yeast DNA to get some transformants?
> 
> Yeast DNA preps are often difficult to transform into coli. My lab 
> has had great success using electroporation of E. coli as our
> transformation method of choice with yeast DNA> 
> susan


I agree - electroporation is the method of choice (especially for a
cen-based vector).  We had little trouble isolating clonable DNA from YPD
(no selection) cultures.

-- 
Colin MacDiarmid
macdiarm at sbsu1.auckland.ac.nz



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