Purifying yeast proteins using six his tag.

[Sherif Abouelela]

Hi to all the yeast lovers:

	Does anybody know of a good way to avoid non specific binding of
proteins to the Ni-NTA column when purifying yeast protein containing a
six his tag. I can see my protein using immunoblotting, but lots of stuff
appear when I stain. Any ideas or tricks


Sherif Abouelela