Yeast expression vector

elsasser at elsasser at
Mon Mar 27 02:43:34 EST 1995

In article <3kmph8$92h at>, T. S. Pillay <tpillay at> writes:
>Dear netters
>Has anyone used yeasts to overexpress proteins (on a preparative scale)
>that are either unstable in or toxic to bacteria.  Is it better than the
>baculovirus system?


Clay Brown developed a bacterial expression vector specifically for proteins
toxic to E.coli.  The reference for this plasmid is GENE 127, 99-130 (1993).
The plasmid, pT7-7 may be commercially available, and should be available by
contacting Judy Campbell, in whose lab it was developed.

Suzanne Elsasser

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