Total DNA isolation
bkrett at ACNS.ColoState.EDU
Wed Mar 29 14:03:34 EST 1995
Someone wrote a letter requesting a protocol for total yeast
isolation using zymolyase. This is definitely a method of extracting the
DNA but I have been using a faster and eaiser method to obtain the same
Adding glass beads and phenol in a tube along with the cells and
vortexing for a total of 3' alternating every 30 sec. on ice works
extremely well for me. By the way this is the method that I use to
isolate total cell RNA tool. (Of course using 5M LiCl instead of NaOAc)
Using the zymolyase works as well as a more gentle method. I use
the Zymolyase to isolate the nuclei for invivo work.
If you have any questions e-mail me for more info.
bkrett at holly.colostate.edu
Colorado State University
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