HELP! PCR using pfu
John P. Aris, Ph.D.
johnaris at anatomy.med.ufl.edu
Wed May 10 04:59:59 EST 1995
I have been using pfu DNA polymerase to amplify a 3-kb fragment from yeast
genomic DNA. I tried different conditions:
94 oC 1.5 min
45,50,55,60 oC 2 min
72 oC 6,7,8 min
10 and 100ng template
2.5 and 5 units
for a total of 50ul reaction. Each time I used taq polymerase as a control.
I could obtain a 3-kb product from taq but nothing from pfu. If anyone knows
how pfu works, would you please tell me about it? Thanks.
Anatomy and Cell Biology
University of Florida
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