Pichia precipitation problem

Lanes olavl at fagmed.uit.no
Tue Nov 7 13:04:45 EST 1995


I have expressed a serine protease with Pichia pastoris using the pPIC9 
vector. The problem is that all proteins in the medium (BMM medium as 
described in the Pichia pastoris expression kit) precipitate. The precipitate
is very difficult to solute and will be a big problem after upscaling the 
expression.

What can I do to avoid this ? Please come up with some good suggestions, 
I have to purify the protein before christmas !

Olav Lanes
Department of Biotechnology
University of Tromsoe, Norway




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