Pichia precipitation problem

Solange S de Camargo scamargo at usp.br
Fri Nov 10 10:26:11 EST 1995


On Tue, 7 Nov 1995, Lanes wrote:

> I have expressed a serine protease with Pichia pastoris using the pPIC9 
> vector. The problem is that all proteins in the medium (BMM medium as 
> described in the Pichia pastoris expression kit) precipitate. The precipitate
> is very difficult to solute and will be a big problem after upscaling the 
> expression.
> 
> What can I do to avoid this ? Please come up with some good suggestions, 
> I have to purify the protein before christmas !
> 
> Olav Lanes
> Department of Biotechnology
> University of Tromsoe, Norway
> 
Hi Olav, 

	Why don't you use a separation column before the preciptation? A 
rapid separation by filtration can help you so much. 

	Good luck

	Solange



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