2-hybrid bait alone activation
kalch at ulam.generes.ca
Mon Nov 13 03:21:38 EST 1995
I have a found a wierd occurence of my YTH bait plasmid: When I do a lacZ
filter assay on yeast harbouring my bait alone plasmid from YPAD plates,
my bait plasmid turns blue alone. However, when this same clone is plated
onto SC dropout media, it FAILS to turn blue.
Does anyone have a comment.
BTW, I am in the vector pGBT9 (-Trp marker)
More information about the Yeast