2-hybrid bait alone activation

Michael kalchman kalch at ulam.generes.ca
Mon Nov 13 03:21:38 EST 1995


Hi there,

I have a found a wierd occurence of my YTH bait plasmid:  When I do a lacZ
filter assay on yeast harbouring my bait alone plasmid from YPAD plates,
my bait plasmid turns blue alone.  However, when this same clone is plated
onto SC dropout media, it FAILS to turn blue. 

Does anyone have a comment.

BTW, I am in the vector pGBT9 (-Trp marker)

Thanks,

kalch



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