DRHOADS at MERCURY.UARK.EDU
Tue Nov 14 17:42:06 EST 1995
> To: yeast at net.bio.net
> From: djdlab at bimcore.emory.edu (Dean Danner Lab)
> Subject: Yeast Introns??
> Date: 14 Nov 1995 18:21:01 GMT
> Reply-to: djdlab at bimcore.emory.edu
> I have recently cloned a PCR product obtained using yeast genomic DNA
> as the template. The product was produced using degenerate primers,
> designed to match sequences conserved across species in a particular
> protein. Some of the sequence seems to match well with the expected
> translated product, however, the clone does not appear to have a single
> ORF (i.e., when translated in the 3 frames suggested by the assumed
> orientation of the original primers, none of the 3 frames are a complete
> One of the possibilities is that this is, indeed, the gene I'm looking for,
> but it contains at least one intron.
> I don't know much about introns in yeast nuclear genes. Basically I want
> to know how to spot an intron from the primary sequence and where to find
> basic info on yeast nuclear genomic introns and splicing.
> I will post a summary of the best help I have received in response.
> Brett Burkholder
> Emory University
> Genetics and Molecular Medicine
You should be able to recognize the GT..AG boundaries and the AG
boundary should be preceded by a lariat site. The hardest part is
recognizing the 5'donor end as the patterns are less specific.
||Doug Rhoads || Dept. of Biological Sciences||
||drhoads at mercury.uark.edu || 601 Science Engineering ||
||drhoads at uafsysb.uark.edu || University of Arkansas ||
||501-575-3251 || Fayetteville, AR 72701 ||
|| My Dogma Just Got Run Over by Someone's Karma ||
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