IF with anti-(His)6

Steven A. Haney shaney at watson.princeton.edu
Tue Nov 14 19:58:49 EST 1995


Hello,

I am interested in both purifying a protein of interest and in doing some 
indirect immunofluorescence in yeast.  There are some places selling anti-
(His)6 antibodies for doing westerns, and I am curious if anyone has tried 
using these antibodies for IF.  If this is feasable, it would allow me to 
get two experiments done with one construct.  

Any experiences?

Thanks,

Steven A. Haney
Broach Lab
Princeton University

shaney at watson.princeton.edu



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