Yeast Introns??

[Ken Howe]

David,
it's not necessarily true that running a yeast sequence through a program 
designed to search for intron-containing ORFs in metazoans will yield the 
appropriate information.  Yeast and metazoan introns differ in many ways- 
regardless of the similarities in their removal and the components of 
machinery by which they are removed.

Besides differences in the frequency and location of introns in PolII 
transcripts between the two groups, splicing signals in yeast are much 
more highly conserved than metazoan signals and even the consensus 
sequences are different:

yeast:   5'exon nn:GUAUGU------------UACUAAC-----------yAG:n 3'exon
metazoan:5'exon AG:GUrAGU------------ynyUrAY--(Py)-----yAG:G 3'exon

In addition, the lengths between these signals varies.  For a computer 
program designed specifically for locating introns in yeast sequences, 
look up a recent paper by C. Fondrat and A. Kalogeropoulos in Current 
Genetics (1994) V25:396-406.

Ken Howe
"The onions expressed here are my own"

 > the following URL 
may be helpful > http://dot.imgen.bcm.tmc.edu:9331/gene-finder/gf.html
> 
> It contains programs for intron/exon boundary identification specifically
> for Drosphila, Arabidopsis and nematodes.  I bet if you ran your sequence
> through these programs it could identify potential intron(s) in your yeast
> gene.
> 
> -- 
> David R. Nelson
> Assistant Professor
> Dept. of Biochemistry
> University of Tennessee, Memphis
> 
>