Quantification of mRNA on Northern Blots
John S. J. Anderson
jacobs at azstarnet.com
Mon Nov 20 09:48:14 EST 1995
In article <48pfa4$fiq at mserv1.dl.ac.uk>, TOMASKA at fns.uniba.sk wrote:
> My colleage is studying the effect of heat-shock on
>transcription of her gene. As a standart for quantification of mRNA
>on membrane she tried to use mRNA for actin. Unfortunately, the
>activity of actin gene seems to be also affected by the heat
>treatment. Can somebody suggest:
> 1. Another acceptable standart mRNA?
Our lab uses the 7S rRNA for quantification purposes. I'm 90% positive
that it isn't affected by heat shock.
> 2. A possible source of a probe?
Here, I don't know. We end-label an oligo we had sythnesized.
> Thank you in advance for all suggestions.
Sure, let me know if you have any more questions.
> Lubo Tomaska tomaska at fns.uniba.sk
> Department of Genetics
> Comenius University
> Faculty of Natural Sciences
> Mlynska Dolina B-1
> 842 15 Bratislava
jacobs at azstarnet.com (<-- this is my 'home' address)
Dep't of Mol & Cell Biol
University of Arizona
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