Gal1 promoter construct of mine
Jon Stewart
jds2 at ufcc.ufl.edu
Wed Nov 22 15:24:08 EST 1995
Colin,
I'm glad to hear that someone else has seen this phenomenon and that we
are not just crazy! We have been using pYES2 to express a monooxygenase
and have found that in glucose, the enzyme was expressed at ca. 50% of
the level achieved when the yeast were grown on 2% galactose. Good
thing we're not expressing a toxic gene product!
Jon Stewart
Department of Chemistry
University of Florida
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