suppressor of null alleles

Sepp D. Kohlwein kohlwein at fscm1.dnet.tu-graz.ac.at
Mon Nov 27 06:07:16 EST 1995


<linder at cmu.unige.ch> wrote:
>
>Dear Netters,
>
>does anybody has tried once to isolate suppressors of a null allele? To
>find parallel pathways. To rescue similar proteins for the deleted
>function?
>
>I would greatly appreciate any comments or references.
>Patrick
>
>
>
>
>***********************************
>Patrick Linder
>Department of medical Biochemistry
>CMU
>1, rue Michel Servet
>1211 Geneve 4
>tel +41 22 702 54 84
>fax +41 22 702 55 02
>linder at cmu.unige.ch
>

Dear Patrick,

Vytas Bankaitis has isolated suppressors of sec14ts, which also suppress
the (lethal) sec14::knockout. There are some 6 suppressors (point 
mutants or deletions) which, in combination with sec14::knockouts render 
cells viable (and without a secretion phenotype...).
Check Cleves et al. 1991, Cell 64:789-800

Regards,

Sepp D. Kohlwein


***********************************************************************
Sepp D. Kohlwein, PhD              
Genetics and Molecular Biology Group
Department of Biochemistry and Food Chemistry
Technical University Graz                   phone:  ++43 (316) 873-6456
Petersgasse 12                              fax:    ++43 (316) 873-6952
A 8010 Graz, Austria           e-mail kohlwein at fscm1.dnet.tu-graz.ac.at
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