luciferase for yeast?

Peter Russell yeast at reed.edu
Wed Oct 4 19:30:11 EST 1995


Wolfgang Fecke wrote:
>are there any reporter genes available (apart from the common
b-galactosidase/X-gal system) which do NOT select for growth (like HIS3
or LEU2)? Does anyone have experience with the luciferase gene in yeast?
I am studying transcriptional activation with the lacZ gene in yeast and
would like to have a good control.

----

We have done some work with firefly luciferase in yeast - see NARes
19:4949-4953 (1991).  The paper describes experiments to test the
feasibility of doing RNA transfection experiments.  More recently
we have expressed luciferase from a YCp and a YEp based plasmid
vector.  Transient luciferase expression in yeast can also occur
from plasmids with the firefly luciferase gene under the control
of the CaMV 35S promoter.

Peter Russell
yeast at reed.edu





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