Q: Embedding in Lowicryl K4M for TEM

Vladislav Raclavsky rac at risc
Wed Oct 4 12:16:16 EST 1995


	Dear colleagues,

	   I would greatly appreciate any comments on Lowicryl K4M embedding
of yeast cells for labelling with WGA-lectin 10 nm Colloidal Gold. We use a
procedure described in the Lowicryl K4M manual but the cells often separate
from the embedding medium in ultrathin sections and fall out. If the
suspension isn't very dense it's working better but it still isn't
satisfactorily for good documentation.
	   Thank you very much, you can mail either to this group or to the
e-mail adress: rac at risc.upol.cz

Vladislav Raclavsky, M.D.
Dept Biology, Fungal Research Lab
Univ Palacky School of Medicine
CZ-775 15 Olomouc
Czech Republic



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