sporulation of s288c diploid

[Karen Sirum-Connolly]

Dear Colin and Dave,

		The sporulation problem in s288c could be due to one of those "common 
markers", namely his3-delta200.  If the diploid is homozygous for this 
allele, it may not sporulate well due to reduced expression of PET56.  
Check the bionet.molbiol.yeast archives for a discussion of PET56 and 
his3-delta200 that occured in August.  I would be happy to send you 
PET56 on a plasmid to see if this helps.  Marc Garten told me this 
solved his sporulation problems.

Best Regards,

Karen

Karen Sirum-Connolly
Dept. of Biochemistry and Molecular Biology
University of Massachusetts
Amherst, MA 01003
phone 413-545-0469
fax 413-545-3291
email ksirum at biochem.umass.edu