luciferase for yeast?
bxg00676 at niftyserve.or.jp
Sat Oct 7 12:25:23 EST 1995
<Pine.SUN.3.91.951002175825.9376A-100000 at konichiwa.cc.columbia.edu>,
Wolfgang Fecke <wf14 at columbia.edu> wrote:
> are there any reporter genes available (apart from the common
> b-galactosidase/X-gal system) which do NOT select for growth (like HIS3
> or LEU2)?
CAT works fine in yeast -- extremely sensitive. Check out a paper by Griggs
& Johnson in MCB (regulation of the GAL4 promoter) from sometime around
1991 - 92.
If you want more info or vectors contact me at lamphier at po.infosphere.or.jp
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