S-35 metabolic labeling overnight

[Darlene Falvey]

I am in need of a protocol for labeling yeast overnight with TranS35Label 
(ICN). Previously, I have done six hour labelings with a final activity 
of 50 uCi/ml but have not been able to isolate a labeled protein by IP.  
It has been suggested that the turnover of our protein of interest might 
be too long and a longer labeling might help. If anyone has any 
experience with overnight labeling of yeast cultures with S-35, I would 
appreciate any insight you could provide.