Low Phosphate Media?

Marek Tchorzewski TCHORZ at BIOTOP.UMCS.LUBLIN.PL
Wed Oct 11 06:25:44 EST 1995


>Dear Yeast Researchers,
>
>     I would like to radiolabel yeast cells with 32-P phosphate. I have 
>seen references to low phosphate media in the literature, but I can't 
>seem to find it in the catalogs. I am using YNB in a SR media. Difco 
says 
>that they don't have anything more specific. 
>
>     What are people using for low phosphate media and where can I 
buy 
>some? 
>
>     Thank you very much in advance,
>
>     Jonathan Mazer (jsmazer at ucdavis.edu)

Hello Jonathan,

We used Retel and Plant's mediun for in vivo labelin with 32-P
with quite good results

Marek



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