Vector stability

Daniel Harkness harkness at
Wed Oct 11 09:57:25 EST 1995

Hello All,

Does anyone have any information regarding the maximum number of plasmids
one can transform into S. cerevisiae?  I have a trp-, his-, ura-, leu-
strain and would like to transform 4 constructs that carry the 4 different
markers and of course select on u-, t-, l-, h- medium.  Is there anything
I should worry about?  The vectors are 2 expression (CEN and 2um), 1 cDNA 
library (2um), 1 reporter (CEN).

Dan Harkness
Boston University
Dept. of Cell and Molecular Biology
5 Cummington St.
Boston, MA 02215
email: harkness at

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