harkness at bio.bu.edu
Wed Oct 11 09:57:25 EST 1995
Does anyone have any information regarding the maximum number of plasmids
one can transform into S. cerevisiae? I have a trp-, his-, ura-, leu-
strain and would like to transform 4 constructs that carry the 4 different
markers and of course select on u-, t-, l-, h- medium. Is there anything
I should worry about? The vectors are 2 expression (CEN and 2um), 1 cDNA
library (2um), 1 reporter (CEN).
Dept. of Cell and Molecular Biology
5 Cummington St.
Boston, MA 02215
email: harkness at bio.bu.edu
More information about the Yeast