Protein tags in yeast

MARK BOSSIE bossiem at pt.cyanamid.com
Thu Oct 12 11:37:58 EST 1995


Try the c-myc tag and MAb 9E10.  It has been used in yeast with 
a good deal of success and I've never had a significant background
problem. 

Regards,
M. Bossie

In article <Pine.OSF.3.91.951005145202.24701A-100000 at duke.usask.ca>, Todd Hryciw <toddh at duke.usask.ca> writes:
> Does anyone know of a good protein tag for use in cerevisiae? I have 
> heard that HA gives a high background. ( I wish to localize my tagged 
> protein in the cell by Western blots.)
> Thanks,
> Todd Hryciw
> Dept. of Microbiology
> University of Saskatchewan, Canada



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