size of yeast

[Sepp D. Kohlwein]

oleh at post.its.mcw.edu (Oleh Kovalchuke) wrote:
>Haploid - 70 um.
>Diploid - 120 um.
>1 cell - 60 (80) pg.
>From Sherman's paper in Meth. of Enzymol.
>
>Oleh


I guess a decimal point is missing here.... 7.0 (1n) or 12.0 (2n) 
microns would be closer to S.cerevisiae non-pseudo hyphal cell size...

mamber at synapse.bms.com (Stephen Mamber) wrote:
>In article <ucecegv.23.0083ABA4 at ucl.ac.uk>, ucecegv at ucl.ac.uk (Edward Varga) says:
>>In article <debbritt-111095112455 at cis-ts3-slip8.cis.brown.edu> debbritt at brownvm.brown.edu (Deb Britt) writes:
>>>A quick question, since I haven't found the answer in any of my texts yet,
>>>what is the average size of a Saccharomyces cell?
>>I think  it's between about 4 to 7 micron depending on the strain & how it's 
>>grown. 
>>
>I don't have the book in front of me but I think it's about DOUBLE your
>estimate.
>
>SWM
>
>mamber at synapse.bms.com

We have done extensive measurements by CLSM and the first reply was
about correct. pet strains are about 4 um and haploid wild type (w303,
D273-10B) is in the range between 7 to 8 microns; diploids are about
8 to 10 um.
Wished the cells were a little bigger for studying subcellular structures by
light microscopy... (sigh...)

Sepp D. Kohlwein




***********************************************************************
Sepp D. Kohlwein, PhD              
Genetics and Molecular Biology Group
Department of Biochemistry and Food Chemistry
Technical University Graz                   phone:  ++43 (316) 873-6456
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A 8010 Graz, Austria           e-mail kohlwein at fscm1.dnet.tu-graz.ac.at
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