quick Western and HA Ab

Tomas Drgon tomasd at bdg10.niddk.nih.gov
Wed Oct 18 08:50:32 EST 1995


In article <gbutler-181095091433 at gbutlermac.ucd.ie>,
gbutler at macollamh.ucd.ie (Geraldine Butler) wrote:

> In article <tomasd-0609560945320001 at fp5-9n.domain>,
> tomasd at bdg10.niddk.nih.gov (Tomas Drgon) wrote:

> > I use mouse antiHa from Boehringer (cat#1583 816), 1:10000 for 30 min at
> > RT, 2-nd antibody goat anti-mouse coupled w HRP, nickel enhanced

> > Tomas Drgon
> > 
> > -- 
> > Signature under construction...
> 
> 
> Hi, 
> I'm interested in the reference to the Boehringer antiHA antibody, at a
> dilution of 1:10000.  The instructions with this antibody say to use 5ug/ml
> - as they supply 200ug in a standard pack, this doesn't allow many
> westerns! Have many people tried it at dilutions such as 1:10000?
> Thanks
> -- 
> Geraldine Butler  University College Dublin   Ireland

Sorry, sorry, my fault.  I mixed up things. I also use the Boehringer
antiHa at 5 ug/ml, overnight in the coldroom. 
Thanks for correction, and sorry again.

Tomas Drgon

-- 
Signature under construction...



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