Need recipe for low pH YPD plates
Solange S de Camargo
scamargo at usp.br
Fri Oct 20 05:44:13 EST 1995
I think you should make the agar solution at 3.0 % (2-fold
concentrated) alone. Autoclave it and then mix to the YP medium,
also previously autoclaved and previously prepared at the
desired pH. Then, you should homogeneize it and add dextrose.
I guess your problem is because the low pH and the high
temperature are hydrolizing the proteins of the agar.
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