colony PCR

Hoffmacs Hoffmacs at HERMES.BC.EDU
Fri Oct 20 06:12:02 EST 1995


Dear Kent et al.,
We have been amplifying products up to 2 kb from S. pombe using a small
portion of a freshly grown colony in a standard PCR reaction.  While we often
start with Mg++ curve, our typical result is that no additional Mg++ is
required beyond that in the 10X buffer.  We use VENT, so I don't know if Taq
or Pfu users have a different experience.  Good luck.
Charlie Hoffman
"I'd rather be fission."
________________________________________________________


Does anyone have info regarding colony PCR with S. cerevisiae when 
expected products are 1000 bp or more?





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