Need recipe for low pH YPD plates

Kevin Morano kamorano at ucdavis.edu
Thu Oct 19 13:17:46 EST 1995


Hi Louis,

In our lab, we routinely make pH 5.5 plates and our method is this:

1.  Autoclave the agar, Y and P.
2.  Make concentrated buffer and glucose, autoclave separately.
3. Add together after cooling, pour plates.

If your buffer is strong enough, it shouldn't be affected by the pH of the
YP mix.  I don't know if this would work for pH 2.5.  We found that the
agar liquefaction process only occurred when the agar was autoclaved at
the low pH, not when pH'd after sterilizing.  Good luck.

Kevin Morano
Klionsky lab
UC Davis Microbiology



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