competent cg1945 for 2hybrid

Keith Gough Keith.Gough at mel.dbe.csiro.au
Tue Oct 31 18:17:05 EST 1995


G'day to all,

Does anybody have "the ultimate" method to obtain high efficiency competent 
CG1945  yeasts for the 2 hybrid system. I am doing a sequential 
transformation rather than co-transformation. I cannot, so far, obtain a 
transformation efficiencies of > 10 to the 3 or 4 and, as a result, I am 
forced to do many transformations. I am following the method descibed in the 
Matchmaker protocol pamphlet.

Thanks Keith



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