His-tag purification of multi-subunit protein
JBEGGS at SRV0.BIO.ED.AC.UK
Fri Sep 1 03:26:25 EST 1995
Mike McAlear wrote:
I have a question concerning the His-tag system for purifying
proteins from yeast. I am thinking of trying to purify a multi-subunit
protein from yeast by tagging one of its subunits with the 6 x His tag.
Has anyone tried this approach? I am concerned about whether or not the
other subunits will stay associated through the purification steps. I
realize that different proteins will likely behave differently, but any
info would be appreciated.
Try contacting Debra Wiest in John Abelson's lab who recently reported
at an RNA Processing meeting that this was successful for isolation
of the Prp9/21/11 complex.
Professor Jean D. Beggs
Institute of Cell and Molecular Biology
University of Edinburgh
Edinburgh EH9 3JR
Tel: 44 131 650 5351
Fax: 44 131 668 3870
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