Detecting GFP colonies

Juana janton at facstaff.wisc.edu
Fri Sep 8 14:13:22 EST 1995


I have expressed GFP (the S65T mutant; excite/emit= 490/510 nm) in yeast 
but was not able to distinguish expressing from non-expressing yeast 
colonies on plates. However, my level of expression might be very low. I 
would like to know if somebody has been able to distinguish GFP 
expresing yeast colonies on plates? Is there a critical level of 
expression to visually detect expression?
Partial answers welcome.

Juana





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