Detecting GFP colonies
janton at facstaff.wisc.edu
Fri Sep 8 14:13:22 EST 1995
I have expressed GFP (the S65T mutant; excite/emit= 490/510 nm) in yeast
but was not able to distinguish expressing from non-expressing yeast
colonies on plates. However, my level of expression might be very low. I
would like to know if somebody has been able to distinguish GFP
expresing yeast colonies on plates? Is there a critical level of
expression to visually detect expression?
Partial answers welcome.
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