Detecting GFP colonies

Michael Moser moser at U.WASHINGTON.EDU
Fri Sep 8 15:18:34 EST 1995


Dear Juana
	I am unable to detect any fluorescence from yeast colonies
expressing fusions to either GFP or S65T GFP.  When I examine my strains
using a fluorescence microscope, fluorescence of wt GFP is faint but
detectable, S65T is easily visible.  My constructs are not over-expressed.
Sincerely,

Mike Moser                                             Tel: 206-543-5354
UW Department of Biochemistry                          FAX: 206-685-1792
Box 357350                                        moser at u.washington.edu
Seattle, WA  98195-7350                     Make peace my beast is yeast




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