Detecting GFP colonies

Sandy Silverman sandy at icr.pt.cyanamid.COM
Mon Sep 11 16:05:23 EST 1995


I have tried GFP under the TDH promoter and got very bright fluorescence in
colonies.  This is a strong promoter, so this may be the explanation.  Also,
remember that you have to use long wave UV so a transilluminator is not 
optimal (I forget the best excitation wavelength but it is closer to blue 
than to UV.)
------Sandy
--
Sanford Silverman                   >Opinions expressed here are my own<
American Cyanamid  
silvermans at pt.cyanamid.com     			"Yeast is Best"



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