need help on gal1 promoter induction

Tao Wei tw26 at cornell.edu
Mon Sep 11 15:08:03 EST 1995


Dear netters:

I've constructed tomato cDNA library in pYES2 (Invitrogen) using gal1
promoter to drive the expression of cDNAs.  Can I induce the expression by
growing yeast cells on plates having both dextrose and galactose? Or can
you tell me what is the best ratio of galactose to glucose in the medium
which not only supports yeast growth but also induces the expression from
gal1 promoter.  Thanks in advance.



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