b-Gal assay / yeast

Kristin B. Andersson k.b.andersson at labmed.uio.no
Thu Sep 14 03:22:26 EST 1995


As a newcomer to yeast, I was wondering what is currently the best
protocol for doing b-gal assays in yeast:

          a) filter colonies  (other than dipping in N2)

          b) suspension.


A b-gal method for gram- bugs was recently described in Biotechniques
using polymyxin B to permablilize the cell wall.  Has anyone tried
anything similar for yeast??

Thankfull for any advice

Kristin

-- 
Kristin B. Andersson                      k.b.andersson at labmed.uio.no
University of Oslo
Institute for Biochemistry                Phone:  +47-2285-6699
PB 1041 Blindern                          Fax:    ?47-2285-4443
N-0316 Oslo, Norway



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