glass beads for yeast lysis

bicfo at bicfo at
Tue Sep 19 00:53:11 EST 1995

hallo all!
Can someone give me the good oil on using glass beads to lyse yeast? I'm using 
1ml of lysis buffer (50mM HEPES, pH 7.4, 150mM NaCl, 5mM EGTA, 5mM EDTA, 2% 
Triton x-100 and inhibitors) to ~ 6 x 10^8 cells and 1.5g 400 -600u 
acid-washed beads. I'm vortexing them to death - and yet I'm getting zilch in 
the way of lysis! What am I failing to do?
Replies gratefully received!
Cheers, Colleen

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